Document 0071
 DOCN  M9490071
 TI    Removal of the cleavage site of recombinant feline immunodeficiency
       virus envelope protein facilitates incorporation of the surface
       glycoprotein in immune-stimulating complexes.
 DT    9411
 AU    Rimmelzwaan GF; Siebelink KH; Huisman RC; Moss B; Francis MJ; Osterhaus
       AD; Department of Virology, Erasmus University Rotterdam, The;
       Netherlands.
 SO    J Gen Virol. 1994 Aug;75 ( Pt 8):2097-102. Unique Identifier : AIDSLINE
       MED/94322005
 AB    Recombinant vaccinia viruses were constructed that expressed the
       complete env gene of feline immunodeficiency virus with or without the
       nucleotide sequence encoding the cleavage site between the surface (SU)
       protein and the transmembrane (TM) protein. The removal of this cleavage
       site resulted in the expression of a 150K protein that was processed
       into a 130K protein and was not cleaved into the SU and the TM proteins.
       Removal of the cleavage site also facilitated incorporation of the SU
       protein in immune-stimulating complexes (iscoms). Antibody responses to
       both an SU and a TM peptide representing two immunodominant B cell
       epitopes were measured. These were higher in cats immunized with iscoms
       prepared from the cleavage site-deleted envelope protein than in cats
       immunized with iscoms prepared from the native envelope protein or
       immunized with the envelope protein and the adjuvant Quil A.
 DE    Animal  Antibodies, Viral/*BIOSYNTHESIS  Antigens,
       Viral/GENETICS/*IMMUNOLOGY  B-Lymphocytes/IMMUNOLOGY  Base Sequence
       Cats  Feline Acquired Immunodeficiency Syndrome/PREVENTION & CONTROL
       Gene Products, env/GENETICS/*IMMUNOLOGY  Immunodeficiency Virus,
       Feline/GENETICS/*IMMUNOLOGY  Immunodominant Epitopes/IMMUNOLOGY
       Molecular Sequence Data  Protein Processing, Post-Translational/GENETICS
       Recombinant Proteins/IMMUNOLOGY  Vaccines, Synthetic/IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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